Summary of Brucella ovis ATCC 25840, version 29.0

Tier 2 Curated Organism Database

Database Authors

Lisa Moore¹, Amanda Mackie¹, Ron Caspi², Suzanne Paley², Anamika Kothari², Ian Paulsen¹, Peter D Karp²

¹Macquarie University, ²SRI International

Summary

The alpha-proteobacteria genus Brucella contains dozens of species, all of which are animal pathogens. New species continue to be discovered, some of which, like Brucella abortus, Brucella suis and Brucella melitensis, are known to cause the zoonotic disease, brucellosis, in humans. However, Brucella ovis is not known to cause disease in humans; rather, it is a highly contagious, veterinary pathogen causing genital disease in sheep that can manifest as epididymitis, ovine infertility, placentitis, abortion and neo-natal fatality. Sheep and deer are known reservoirs, though some families within the Bovidae may also be susceptible to ovine epididymitis. There are other microbes, such as B. melitensis, Actinobacillus seminis and Histophilus somni (previously known as H. ovis), that are also known to cause ovine epididymitis [Buddle56, Burgess82, Godfroid11, EFSA17].

B. ovis is found worldwide in all countries with a sheep industry, with B. ovis infections being a major economic problem. Vaccination against the disease is an important alternative to culling infected herds, and research into prevention, such as new vaccines, continues [Carpenter87, Carpenter87a, GarinBastuji98, Buddle56, Burgess82, Godfroid11, Martins12, EFSA17].

Brucella species are facultative, intracellular pathogens that get incorporated into a brucella-specific vacuole within the lysosome of host cells. However, there are distinct differences in virulence between different Brucella species in addition to differences in host specificity. One of the major virulence factors of most Brucella, such as Brucella abortus, is the O-polysaccharide chain in the lipopolysaccharide (LPS), which results in a "smooth" phenotype. B. ovis, on the other hand, is considered naturally "rough" due to the absence of the LPS O-chain, due to a combination of pseudogenes and deletions in various outer membrane protein (OMP) genes, such as Omp25b which is required for the synthesis of the O-polysaccharide chain found in smooth Brucella spp. Metabolic differences also exist, such as the inability of B. ovis to grow on glucose, galactose or erythritol as primary carbon sources, a negative oxidase phenotype, and lack of nitric oxide reductase, some of which contribute to it's restricted host range [Vizcaino04, Moreno14, Tsolis09, Roop09, He12].

B. ovis strains possess a species-specific genomic island, named the "Brucella ovis pathogenicity island 1" (BOPI-1), that is associated with pathogenesis [Tsolis09, Alvarez16]

The genome used for this Pathway/Genome Database (PGDB) was sequenced in 2007 from a monoisolate of the type strain isolated from sheep tissue in Australia in 1960. The genome contains two chromosomes like other Brucella genomes. This Pathway/Genome Database (PGDB) was generated by the PathoLogic [Karp16, Karp11] component of Pathway Tools software version 24.5 and MetaCyc [Caspi18] version 24.5 on 04-Feb-2021 14:29:24.

Genome

Replicon	Total Genes	Protein Genes	RNA Genes	Pseudogenes	Size (bp)	NCBI Link
Chromosome I	2,064	1,878	49	137	2,111,370	NCBI Assembly:GCF_000016845.1
Chromosome II	1,117	971	17	129	1,164,220	NCBI Assembly:GCF_000016845.1

Total:	3,181	2,849	66	266	3,275,590

Ortholog data available?	Yes

Database Contents

Genes	3,181
Pathways	266
Enzymatic Reactions	1,414
Transport Reactions	58
Polypeptides	2,850
Protein Complexes	116
Enzymes	762
Transporters	266
Compounds	1,032
Transcription Units	2,088
tRNAs	53
Growth Media	1
Protein Features	3,745
GO Terms	25,192
Gene Essentiality Datasets	1

Database Version

29.0

Taxonomic Lineage	cellular organisms Bacteria <bacteria> Pseudomonadota Alphaproteobacteria Hyphomicrobiales Brucellaceae Brucella/Ochrobactrum group Brucella Brucella ovis Brucella ovis ATCC 25840
Genetic Code Number	11 -- Bacterial, Archaeal and Plant Plastid (same as Standard, except for alternate initiation codons)
BIOSAMPLE	SAMN02604024
NCBI BioProject	PRJNA224116
NCBI-Taxonomy	444178
Pathogenicity	animal
Host	Ovis aries
Annotation Provider	NCBI RefSeq
Annotation Pipeline	NCBI Prokaryotic Genome Annotation Pipeline (PGAP)
Annotation Pipeline Version	4.11
Annotation Comment	Best-placed reference protein set; GeneMarkS-2+